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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Virology. 2015 Jul 1;484:288–304. doi: 10.1016/j.virol.2015.06.006

Figure 2. Detection of LC3 and CVB viral protein in shed EMVs.

Figure 2

Differentiated NPSCs transduced with adeno-LC3-GFP were infected with dsRED-CVB (moi = 0.1) and observed by fluorescence microscopy at 3 days PI. (A) Abundant shed EMVs (white arrows) expressing viral protein (red) and a marker for autophagosomes (LC3-GFP, green) were readily observed. (E-F) Higher magnification of (C) showed colocalization of viral protein and LC3-GFP in shed EMVs.