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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Virology. 2015 Jun 20;484:163–169. doi: 10.1016/j.virol.2015.06.003

Figure 2.

Figure 2

Figure 2

Characterization of VP1 protein expression and VLP production by rNDVs in DF1 cells and embryonated eggs. (A) Kinetic analysis of VP1 expression by rNDVs was conducted in DF1 cells and in allantoic fluid of chicken embryonated eggs. Cell culture medium was collected every 12 h and subjected to Western blot analysis. Allantoic fluid was harvested every 24 h post infection and clarified by centrifugation at 3,000 rpm for 10 min for Western blot analysis. Each figure represents three independent experiments. (B) Production of VLPs by rNDVs was analyzed by electron microscope. VLP suspension (10 μ each) was fixed in copper grids, negatively stained with 1% ammonium molybdate, and visualized by using an electron microscope. Baculovirus-expressed VLP in Sf9 cells was included as a control. VLPs expressed by rNDVs were prepared from infected allantoic fluid of chicken embryonated eggs. Each figure represents five independent experiments.