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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Virology. 2015 Jun 6;484:412–420. doi: 10.1016/j.virol.2015.05.011

Figure 7. Gradient stability of 186-TC SINV viral nucleocapsids.

Figure 7

Purified [35S]labeled WT or 186-TC mutant SINV viruses were solubilized in (A) lysis buffer alone or (B) lysates prepared from 186-TC mutant-infected BHK cells that were cultured at 28°C as in Fig. 6 but without radiolabeling. Solubilized virus samples were layered onto sucrose gradients and separated by sedimentation. Fractions were quantitated by scintillation counting, and the percentage of total radioactivity in each fraction was determined. Shown is a representative example of two independent experiments.