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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Virology. 2015 Jun 6;484:412–420. doi: 10.1016/j.virol.2015.05.011

Table 2.

Specific infectivity of 186-TC SINV and 190-TC SFV mutants

Infectivity at 37°C (IC/cpma) Infectivity at 28°C(IC/cpma)
SINV 186-TC SINV WT SINV 186-TC SINV WT SINV
1.2 ± 0.1 3.9 ± 0.2 51 ± 16 82 ± 4
SFV 190-TC SFV WT SFV 190-TC SFV WT SFV
1.6 ± 0.4 3.4 ± 0.7 38 ± 19 34 ± 7
a

Purified [35S]-labeled WT SINV, WT SFV, 186-TC SINV or 190-TC SFV virus stocks were allowed to infect BHK cells for 1–1.5 h at 37°C or 3.5–4 h at 28°C. Further infection was then blocked by addition of 20 mM NH4Cl, and the infected cells were quantitated after incubation at 37°C for 7 h or at 28°C for 16h. Data represent the average and range from two independent experiments, and are expressed as the ratio of infectious centers to virus radioactivity. The somewhat higher infectivity observed at 28 °C reflects the more efficient detection of primary infection with longer time of culture.