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Kinase activation. HeLa cytosol was reacted with recombinant GST or GST-LE in the presence or absence of okadaic acid (OA) and/or DMSO. After 45 min of incubation, the samples were fractionated then probed by Western analyses using (A) α-ERK/12, α-P-ERK1/2, or (B), α-p38, α-P-p38. Pixel normalization for each lane was relative to the respective, unactivated forms (unphosphorylated) of the kinases.
