Table 2. Volumes of the VPg-pU bands and the relative incorporation of P32-UMP into VPgpU/VPgpUpU bands.
The volumes for the VPgpU bands (see Figure 4) are given in total units (to allow comparison from one polymerase to the other) and then relative to the wild type VPg for each 3Dpol (shown bold). Results with the consensus VPg are underlined for each 3Dpol.
| 3Dpol/VPg: | VPg-pU | |
|---|---|---|
| CVA24/CVA24VPg | 54134 | 1.00 |
| CVA24/CVB3VPg | 82568 | 1.53 |
| CVA24/EV A71VPg | 106020 | 1.96 |
| CVA24/PCPconVPg | 99805 | 1.84 |
| CVA24/PV1-VPg | 33164 | 0.61 |
| CVB3/CVA24VPg | 17090 | 0.54 |
| CVB3/CVB3VPg | 31852 | 1.00 |
| CVB3/EV A71VPg | 11040 | 0.35 |
| CVB3/PCPconVPg | 15722 | 0.49 |
| CVB3/PV1-VPg | 13223 | 0.42 |
| EV A71/CVA24VPg | 12208 | 0.25 |
| EV A71/CVB3VPg | 23309 | 0.48 |
| EV A71/EV A71VPg | 48621 | 1.00 |
| EV A71/PCPconVPg | 16776 | 0.35 |
| EV A71/PV1-VPg | 13903 | 0.29 |