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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Inflamm Bowel Dis. 2015 Oct;21(10):2429–2440. doi: 10.1097/MIB.0000000000000512

Figure 2. Distinguishing stricturing Crohn’s-ileitis using eosinophil peroxidase (EPX) immunohistochemistry based scoring system. Eosinophil infiltration correlates with disease activity, chronicity and fibrosis.

Figure 2

EPX immunohistochemistry provides a quantitatively sensitive strategy to distinguish inflammatory from stricturing CD specimens. Examination of the scores for individual EPX parameters associated with all control (CL), inflammatory (INFL) or stricturing (STR) CD patients found in Table 1 demonstrated differences between (A) their overall scores, in addition to individual parameters comprising the EPX algorithm: (B) Patchiness, (C) Degranulation, (D) Peak eosinophils / hpf and (E) average eosinophils across 5 random fields-of-view (F.O.V.). Statistical assessments (ANOVA with Newman-Keuls) of the EPX-staining scores (means ± SEM) demonstrated the utility of this algorithm to distinguish between stricturing and inflammatory CD mucosal specimens. *P≤0.05, **P≤0.01, ***P≤0.001. Peak numbers of ileal eosinophils / hpf (EPX) were analyzed for relationships to subjects’ (F) pediatric Crohn’s disease activity index (PCDAI), (G) histological activity and (H) fibrosis score measured in H&E stained tissues for subjects defined in Table 1 that were not considered under treatment relevant to their CD. The Pearson correlation coefficient (r) and its associated statistical significance are shown.