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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Inflamm Bowel Dis. 2015 Oct;21(10):2429–2440. doi: 10.1097/MIB.0000000000000512

Figure 4. IL-33-activated eosinophils induce pro-inflammatory intestinal fibroblast activation and perpetuation of eosinophil recruitment in vitro.

Figure 4

Confluent 18Co intestinal fibroblasts cells were co-cultured with IL-33 (100ng/ml) and eosinophils. As controls 18Co cells treated with IL-33 alone (100ng/ml), 18Co cells co-cultured with eosinophils in the absence of IL-33 or 18Co cells alone were used. (A) Pro-inflammatory cytokine, (B) eosinophilic chemokine and (C) cytokine receptor mRNA analysis of 18Co fibroblasts was performed by real-time PCR on extracted RNA following 24 and 48 hours of co-culture. In a second series of experiments cells were treated in the 4 groups described above. Following 48 hours of co-culture 18Co cells were washed and IL-13 (100ng/ml) was placed into each of the wells. (D) Fibrosis-associated molecule mRNA analysis of IL-13-stimulated 18Co fibroblasts was performed by real-time PCR on extracted RNA following an additional 72 hours of culture. Data are expressed as means ± SEM of 2–6 individual donors. Statistical significance was assessed using 1-way ANOVA with Newman-Keuls multiple comparisons test. *P≤0.05, **P≤0.01, ***P≤0.001.