Skip to main content
. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Bioorg Med Chem Lett. 2015 Mar 11;25(21):4976–4979. doi: 10.1016/j.bmcl.2015.03.007

Figure 3.

Figure 3

The binding of β-sitosterol and cholesterol to 17β-HSD4 and E-Syt1 in prostate cancer cell lysates. Affinity chromatography was performed using 200 nM and 600 nM concentrations of biotin (b), biotinylated β-sitosterol (s), and biotinylated cholesterol (c) in (A) PC-3 and (B) DU-145 prostate cancer cell lysates (L). Immunoblotting determined that E-Syt1 binds specifically to β-sitosterol, whereas 17β-HSD4 binds more strongly to cholesterol. S5A1 does not bind either compound at the tested concentrations.