Table 3.

Proteins involved in carbohydrate transport and metabolism were secreted into control medium supplemented with either microcrystalline cellulose or microcrystalline cellulose and suberin

UniProt accession number	Corresponding gene in the S. scabiei 87.22 genome	Putative function	CAZy classification	Normalized spectral abundance factor (%)
				CM+C	CM+C+S
Cellulases
C9YVP5	SCAB_5981	Cellulase B precursor CelB	CE1	Da	0.94
C9Z0D5	SCAB_8871	Cellulase	CBM13	0.12	0.38
C9ZD50	SCAB_16431	Cellulase CelA1	GH6	1.86	3.06
C9ZEP9	SCAB_17001	Cellulase	GH6, CBM2	D	1.51
C9ZEQ0	SCAB_17011	Cellulase	GH48, CBM2	0.18	1.58
C9ZEQ1	SCAB_17021	Cellulase	GH74, CBM2	NDb	1.47
C9YUZ2	SCAB_36371	Xylanase/cellulase	GH10, CBM2	0.19	1.77
C9YW88	SCAB_37051	Cellulase/xylanase	GH10	0.46	2.06
C9YTK2	SCAB_51081	Cellulase	GH5, CBM2	0.22	0.75
C9ZB17	SCAB_77391	Cellulose 1,4-β-cellobiosidase	NFc	ND	0.22
C9Z351	SCAB_86311	Cellulase	GH5	ND	0.30
C9Z9L5	SCAB_90081	Cellulase B precursor	GH12, CBM2	0.30	D
C9Z9L6	SCAB_90091	Cellulase	GH48, CBM2	0.34	2.38
C9Z9L7	SCAB_90101	Cellulase	GH6, CBM2	0.19	0.48
Other proteins involved in carbohydrate transport and metabolism
C9ZBE6	SCAB_0631	α-l-fucosidase	GH29, CBM13	ND	0.13
C9YYV2	SCAB_3881 or SCAB_22931	Arabinofuranosidase	GH62, CBM13	ND	0.68
C9YUC5	SCAB_4961	Glucuronoarabinoxylan endo-1,4-β-xylanase	GH30	ND	0.49
C9YUG2	SCAB_5351	ABC-type sugar transport system	NF	1.27	0.71
C9YVN3	SCAB_5851	Glycosyl hydrolase	CBM32	ND	0.17
C9YVP9	SCAB_6021	Endo β-1,4-xylanase	GH10	0.25	1.37
C9YYN8	SCAB_7551	Glycosyl hydrolase	NF	0.62	D
C9Z1T6	SCAB_9291 or SCAB_91051	Lactonase	NF	ND	0.26
C9Z1U5	SCAB_9381	Exo-α-sialidase	NF	ND	0.53
C9Z507	SCAB_11431	Glycosyl hydrolase	GH43	0.22	1.11
C9Z878	SCAB_13491	Glucan endo-1,3-β-d-glucosidase	GH64	ND	0.13
C9ZD59	SCAB_16521	Arabinofuranosidase	GH43, CBM42	ND	1.81
C9ZD61	SCAB_16551	Mannosidase	GH26, CBM23	ND	0.11
C9YT63	SCAB_19561	β-fructofuranosidase	GH43, CBM13	ND	0.23
C9YUL1	SCAB_19941	Arabinofuranosidase	GH43, CBM42	0.18	0.36
C9YVX8	SCAB_21021	Xylose ABC transporter substrate-binding protein	NF	0.49	ND
C9Z5F4	SCAB_42381	ABC transporter substrate-binding protein	NF	0.26	ND
C9Z5L1	SCAB_42951	Glucose/Sorbosone dehydrogenase	NF	0.93	9.47
C9Z737	SCAB_43661	Galactan endo-1,6-β-galactosidase	GH30, CBM13	D	0.49
C9YY37	SCAB_54441	Enolase	NF	0.18	ND
C9Z2N2	SCAB_57161	Endo-β-1,6-galactanase	GH30	ND	0.21
C9Z451	SCAB_57751	Cellobiose-binding transport system associated	NF	1.46	1.72
C9ZDW4	SCAB_63891	ABC-type xylose transport-system, periplasmic	NF	1.05	0.19
C9ZFW2	SCAB_66021	β-xylosidase	GH43, CBM13	ND	0.32
C9ZFW3	SCAB_66031	Arabinofuranosidase	GH43, CBM42	D	1.09
C9YYF0	SCAB_70591	Pectate lyase	PL9	ND	0.72
C9Z2V1	SCAB_72711	Endo-1,4-β-xylanase	GH11	D	0.62
C9Z2W0	SCAB_72801	Glycosyl hydrolase	NF	ND	1.05
C9Z4J7	SCAB_74141	α-N-furanosidase	GH51	ND	0.43
C9Z623	SCAB_74681	Licheninase	NF	ND	0.44
C9ZAZ8	SCAB_77201	Glycosyl hydrolase	GH106	ND	2.33
C9ZB22	SCAB_77441	α-arabinanase	GH93, CBM13	0.13	0.24
C9ZCR4	SCAB_78891	Glycosyl hydrolase	GH30, CBM13	ND	0.17
C9ZE74	SCAB_79011	Acetyl-xylan esterase	CE2	ND	0.80
C9ZE94	SCAB_79241	Arabinofuranosidase	GH62, CBM13	0.26	1.16
C9ZE95	SCAB_79251	Xylanase A	GH10, CBM13	1.84	10.25
C9ZEC5	SCAB_79561	Glycosyl hydrolase	GHnc, CBM13	ND	0.30
C9YU29	SCAB_82021	β-mannosidase	GH5, CBM2	ND	0.51
C9Z1I5	SCAB_85231	Chitinase	GH19, CBM12	0.44	0.54
C9Z804	SCAB_89741	Cellulose-binding protein	CBM33	0.74	1.05

^aD: detected. Peptides have not fulfilled the filtering criteria as described in the Materials and Methods section.

^bND: not detected.

^cNF: no module has been found in the protein.