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Gelatinase activity of MMP-9 in non-infected cells (WT group, Lane 1, 4), pCMV-Ac-GFP (mock group, Lane 2, 5), and pCMV-Ac-GFP-NDRG2 (NDRG2 group, Lane 3, 6) was measured. Active MMP-9 (82 Kd) degraded gelatin in gelatin-containing SDS-PAGE and left a band that was not stained by Coomassie Blue (A). NDRG2 expression reduced MMP-9 activity compared to the other groups (B). The experiment was performed in triplicate. (*P<0.001).
