FIG 2.
Zur-dependent effects of zinc uptake on V. cholerae growth under zinc-limiting conditions. Overnight cultures of the wild type and zinc transporter mutants were inoculated 1:100 into LB medium (A), LB medium that had been pretreated with 30 μM TPEN (B), and TPEN-treated LB medium with the indicated concentration of ZnSO4 (C). (A and B) The cultures were grown statically at 37°C, and at the time points indicated, the OD600 was measured. Data are the means and SDs from four independent experiments. *, P < 0.05 (Student's t test) compared to the corresponding wild type; ns, no significance compared to the corresponding wild type. (D) The wild type and Δzur mutants containing PznuA-luxCDABE transcriptional plasmid reporters were grown statically in LB medium (none), LB-TPEN medium (−Zn2+), and LB-TPEN medium plus 10 μM ZnSO4 (+Zn2+) to mid-log phase at 37°C. Luminescence was measured and normalized against the OD600. Data are means and SDs from four independent experiments.