FIG 1.

S. mansoni larval migration does not expand Foxp3⁺ Treg cells. C57BL/6 mice were infected with 70 S. mansoni cercariae, and CD4⁺ Foxp3⁺ Treg cells were measured in the lungs, tLN, iLN, and spleen at days 7, 14, and 21 of infection. (A) Representative CD4 and Foxp3 staining of lung CD4⁺ TCR-β⁺ T cells. (B to I) Percentages and numbers of CD4⁺ TCR-β⁺ Foxp3⁺ cells within the lungs (B and C), tLN (D and E), iLN (F and G), and spleen (H and I). Panels show combined data from 3 independent experiments with 4 to 6 mice per group. Symbols represent individual mice, and bars show mean values. Open circles denote results for naive mice, and closed circles denote results for S. mansoni-infected mice.