FIG 2.

Phylogenetic analysis of the FIB replication protein-encoding gene, repA (A), and the FIIA replication protein-encoding gene, repA (B), identified in all AEEC genomes analyzed in this study. The nucleotide sequences were aligned in MEGA5 (37) using ClustalW (38). A maximum-likelihood phylogeny was constructed in MEGA5 (37) using the Kimura 2-parameter model and 1,000 bootstrap replications. All major nodes of the tree are supported by bootstrap values of ≥50 and are indicated on the phylogeny by a circle. The scale bar represents the distance of the number of nucleotide substitutions per site. The symbols next to each genome indicate the presence of the genes for EHEC hemolysin, ehxA, and the major subunit of the bundle-forming pilus, bfpA. The clades in the phylogeny are colored by phylogenomic lineage.