FIG 3.

In silico detection of genes that have similarity to those of the EAF plasmid, pMAR2 (21, 22). TBLASTN (41) BSR (40) analysis of proteins encoded by the EAF plasmid pMAR2 (21, 22) of the EPEC1 lineage prototype isolate E2348/69, compared to the 210 AEEC genomes analyzed in this study, was carried out. Each square represents the BSR of a single gene compared to each genome. Each row is a gene, while each column is a genome. Hierarchical cluster analysis was performed using the Pearson correlation coefficient of MeV (76–78). The colored rectangle above each genome indicates the phylogenomic lineage that each genome belongs to in Fig. 1. The colors of the heatmap indicate the BSRs corresponding to the presence (yellow), divergence (black), or absence (blue) of each of the plasmid genes in each AEEC genome.