TABLE 1.
AEEC plasmids analyzed in this study
| ID | Plasmid size (kb) | No. of plasmids |
BFPa | Phylogenomic lineage | Phylogroup | FIB group | Accession no. | |
|---|---|---|---|---|---|---|---|---|
| ≥20 kb | <20 kb | |||||||
| Isolates | ||||||||
| 303289 | 95.3c | 1 | 3 | + | EPEC1 | B2 | II | JHRJ00000000 |
| 403116 | 95.8 | 1 | 0 | + | EPEC1 | B2 | I | JHTJ00000000 |
| C581-05 | 98c | 2 | 3 | + | EPEC4 | B2 | II | AIBE00000000 |
| 100414d | NDb | 3 | 2 | ± | EPEC4 | B2 | II | JHQX00000000 |
| 401140 | 98c | 2 | 2 | + | EPEC5 | A | II | JHRM00000000 |
| 401091 | 101.6c | 1 | 0 | + | EPEC7 | B1 | I | JHSI00000000 |
| 401588 | 99.4c | 1 | 2 | + | EPEC8 | B2 | II | JHSK00000000 |
| 302053 | 98c | 2 | 2 | + | EPEC9 | B2 | II | JHRG00000000 |
| 100329 | 90c | 1 | 1 | ± | EPEC10 | A | I | JHRT00000000 |
| Previously characterized AEEC plasmids | ||||||||
| pMAR2 (E2348/69) | 97.9 | 1 | 1 or 2 | + | EPEC1 | B2 | I | NC_011603.1 |
| pB171 (B171) | 68.8 | 2 | 1 | + | EPEC2 | B1 | I | NC_002142.1 |
| pO157 (EDL933) | 92 | 1 | 1 | − | EHEC1 | E | II | NC_007414.1 |
| pO103 (12009) | 75.5 | 1 | 0 | − | EPEC2 | B1 | II | NC_013354.1 |
| pO26_1 (11368) | 85.1 | 2 | 2 | − | EHEC2 | B1 | II | NC_013369.1 |
| p101-1 (101-1) | 153.5 | ND | ND | ± | EAEC | A | None | AAMK02000010 |
a
+, presence of all or nearly all of the BFP genes; ±, presence of a few of the BFP genes, including bfpA; −, absence of all of the BFP genes.
b
ND, not determined.
c
Approximate EAF plasmid size of unclosed plasmids based on sequence analysis of the draft genome and by gel visualization of plasmid profiles.
d
EPEC isolate containing an EAF-like plasmid with BFP genes that have ∼72% nucleotide identity to genes of the prototype EAF plasmid pMAR2.