FIG 4.

Cumulative results of immunofluorescence assessment of CdtB internalization and Cdt binding. (A) Results obtained from three experiments for internalization of CdtB in Jurkat cells. (B) Results obtained from three experiments for internalization of CdtB in THP-1 cells. (C) Results obtained from three experiments for holotoxin binding to the cell surface determined by immunofluorescence staining for the presence of CdtC in the absence of permeabilization. Results in each panel are expressed as a percentage of the MCF observed in CdtB^WT and represent the means ± SEM *, P < 0.05, compared to results with the wild-type protein.