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. 2015 Sep 10;83(10):4042–4055. doi: 10.1128/IAI.00788-15

TABLE 1.

CdtB CRAC site mutant constructs

Plasmid Primer Sequence
pGEMCdtBV104P P1 CCGTCCAAATATGCCCTATATTTATTATTCCCG
P2 CGGGAATAATAAATATAGGGCATATTTGGACGG
pGEMCdtBY105P P3 GGTACTCGCTCCCGTCCAAATATGGTCCCTATTTATTATTCCCG
P4 CGGGAATAATAAATAGGGACCATATTTGGACGGGAGCGAGTACC
pGEMCdtBY107P P5 GGTCTATATTCCCTATTCCCGTTTAGATGTTGG
P6 CCAACATCTAAACGGGAATAGGGAATATAGACC
pGEMCdtBR110P P7 GGTCTATATTTATTATTCCCCTTTAGATGTTGG
P8 CCAACATCTAAAGGGGAATAATAAATATAGACC