FIG 7.

SLS promotes keratinocyte cell death through activation of the p38 MAPK pathway. Keratinocytes were treated with p38 inhibitor SB203580 for 2 h prior to infection with GAS. The inhibitor significantly reduced SLS-dependent keratinocyte death as demonstrated by ethidium homodimer membrane permeabilization assay. These data represent the average results of three biological replicates, with error bars representing standard deviations. Statistical significance was determined for each condition compared to control vehicle-treated cells. Overall P values were determined by ANOVA. (A) P < 0.0001. (B) P < 0.0001. (C) P < 0.0001. Dunnett's tests were performed post hoc to compare each condition to the corresponding vehicle control infection (i.e., WT+DMSO).