FIG 1.

L. pneumophila infection causes drastic changes to the cellular PA distribution. A549 cells stably expressing the PA reporter GFP-SPO were left uninfected or infected for 6 h with an L. pneumophila 130b wild type (WT), ΔdotA mutant, or ΔlpdA mutant carrying the empty p4HA or p4HA LpdA plasmid and immunostained using an anti-L. pneumophila antibody (red). DNA was visualized with Hoechst DNA dye (blue). (A) Samples were examined by IF microscopy. Images are representative of at least three independent experiments. Scale bar, 5 μm. (B) Quantification of the effect of infection on the cellular distribution of the GFP-SPO PA reporter by IF counting. Error bars represent the mean standard deviations of three independent experiments.