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Vesicular LpdA localizes to Rab4 (A)- and Rab14 (B)-containing vesicles. HeLa cells were cotransfected with pRK5 HA or pRK5 HA-LpdA and plasmids for the expression of marker proteins of different cellular compartments and processed for IF microscopy. Rab4/Rab14- and LpdA-positive vesicles are highlighted with arrows in the magnified insets. Similar results were observed in at least two independent experiments. Scale bar, 10 μm.
