TABLE 3.

Primers used for site-directed mutagenesis and reverse PCRs in this study

Description/function	Primer sequence (5′–3′)	Source
Introduction of a K165R mutation into LpdA by site-directed mutagenesis PCR	ATAATTCGATTGCGTCCAACCATAATAGAGCCATCTGGATTG	This study
	CAATCCAGATGGCTCTATTATGGTTGGACGCAATCGAATTAT
Introduction of a K376R mutation into LpdA by site-directed mutagenesis PCR	GAAAAAAGAGGTTATTCATATGAGATTTATGTCAATTGATGGGCAGG	This study
	CCTGCCCATCAATTGACATAAATCTCATATGAATAACCTCTTTTTTC
Fusion of a calreticulin ER retention signal to mCherry by reverse PCR	GAGCAGCAGCGGCACGGATAGCAGCATGGTGGCGACCGGTAGGG-CCTTCTTGGCCTAGCAGT	This study
	AGCAGTGAGCAAGGGCGAGGAG
Fusion of a KDEL ER targeting signal to mCherry by reverse PCR	GAGCTGTAACTGATCATAATCAGCCATACC	This study
	ATCCTTTCTAGATCCGGTGGATCCC