TABLE 1.
Strains and plasmids
| Strain or plasmid | Description | Designation | Source or reference |
|---|---|---|---|
| Strains | |||
| TOP10 | E. coli host strain used for propagation of recombinant pIMK2 plasmids | Invitrogen | |
| S17 | E. coli host strain used for conjugation of recombinant pIMK2 plasmids | ||
| NF-L100 | L. monocytogenes 10403S parent strain | L. monocytogenes wild type | 56 |
| NF-L1651 | 10403S with ΔprsA2::erm | L. monocytogenes ΔprsA2 | 33 |
| NF-L3632 | NF-L1651 with integrated pIMK2-L. monocytogenes prsA2 | L. monocytogenes prsA2 | This work |
| NF-L3634 | NF-L1651 with integrated pIMK2-L. monocytogenes prsA1 | L. monocytogenes prsA1 | This work |
| NF-L3636 | NF-L1651 with integrated pIMK2-B. subtilis prsA | B. subtilis prsA | This work |
| NF-L3638 | NF-L1651 with integrated pIMK2-S. pyogenes prsA1 | S. pyogenes prsA1 | This work |
| NF-L3640 | NF-L1651 with integrated pIMK2-S. pyogenes prsA2 | S. pyogenes prsA2 | This work |
| NF-L3642 | NF-L1651 with integrated pIMK2-S. pneumoniae prsA | S. pneumoniae prsA | This work |
| NF-L3644 | NF-L1651 with integrated pIMK2-S. mutans prtM | S. mutans prtM | This work |
| NF-L3646 | NF-L1651 with integrated pIMK2-S. aureus prsA | S. aureus prsA | This work |
| NF-L3648 | NF-L1651 with integrated pIMK2-L. lactis pmpA | L. lactis pmpA | This work |
| NF-L3650 | NF-L1651 with integrated pIMK2-L. monocytogenes prsA2 with a C-terminal 6×His tag | L. monocytogenes prsA2-His | This work |
| NF-L3652 | NF-L1651 with integrated pIMK2-L. monocytogenes prsA1 with a C-terminal 6×His tag | L. monocytogenes prsA1-His | This work |
| NF-L3654 | NF-L1651 with integrated pIMK2-B. subtilis prsA with a C-terminal 6×His tag | B. subtilis prsA-His | This work |
| NF-L3656 | NF-L1651 with integrated pIMK2-S. pyogenes prsA1 with a C-terminal 6×His tag | S. pyogenes prsA1-His | This work |
| NF-L3658 | NF-L1651 with integrated pIMK2-S. aureus prsA with a C-terminal 6×His tag | S. aureus prsA-His | This work |
| NF-L3660 | NF-L1651 with integrated pIMK2-L. lactis pmpA with a C-terminal 6×His tag | L. lactis pmpA-His | This work |
| Plasmids | |||
| pIMK2 | ppL2-derived complementation integration vector | 43 | |
| pNF-3662 | pIMK2 with L. monocytogenes prsA2 | This work | |
| pNF-3664 | pIMK2 with L. monocytogenes prsA1 | This work | |
| pNF-3666 | pIMK2 with B. subtilis prsA | This work | |
| pNF-3668 | pIMK2 with S. pyogenes prsA1 | This work | |
| pNF-3670 | pIMK2 with S. pyogenes prsA2 | This work | |
| pNF-3672 | pIMK2 with S. pneumoniae prsA | This work | |
| pNF-3674 | pIMK2 with S. mutans prtM | This work | |
| pNF-3676 | pIMK2 with S. aureus prsA | This work | |
| pNF-3678 | pIMK2 with L. lactis pmpA | This work | |
| pNF-3680 | pIMK2 with L. monocytogenes prsA2 containing a C-terminal 6×His tag | This work | |
| pNF-3682 | pIMK2 with L. monocytogenes prsA1 containing a C-terminal 6×His tag | This work | |
| pNF-3684 | pIMK2 with B. subtilis prsA containing a C-terminal 6×His tag | This work | |
| pNF-3686 | pIMK2 with S. pyogenes prsA1 containing a C-terminal 6×His tag | This work | |
| pNF-3688 | pIMK2 with S. aureus prsA containing a C-terminal 6×His tag | This work | |
| pNF-3690 | pIMK2 with L. lactis pmpA containing a C-terminal 6×His tag | This work |