FIG 6.

The Rle proteins have distinct subcellular localizations during infection. L. longbeachae NSW150 and the ΔdotB derivative strain were transformed with pMMB207-HA constructs to express Rle proteins with an N-terminal 4×HA tag. These strains were used to infect HEK 293T FcγR cells at an MOI of 1, and the infected cells were fixed and stained at 3 h postinfection with anti-L. longbeachae (red), anti-HA (green), and DAPI (blue). Representative images demonstrate that RleA and RleC associate with the longbeachae-LCV and that RleB is found throughout the cytoplasm in a Dot/Icm-dependent manner. RleD could not be reliably detected above the background anti-HA signal. Scale bar, 10 nm.