Fig. 2.

Effect of the TPF on osteoclast activity as indicated by resorption pit area. Cells were cultured with RANKL (30 ng/ml) and M-CSF (30 ng/ml) for 7 days in the presence of 0, 50 or 100 μg/ml of the TPF and discs were fixed and stained with toluidine blue. a Effects of the TPF on the bone resorbing activity mature osteoclasts and the magnification in a represent ×10, b total resorbed pit area/well and c resorbed pit area/osteoclasts. The data were expressed as the mean ± SD (n = 4) for each group. *p < 0.05