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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Invest Dermatol. 2015 May 7;135(10):2502–2511. doi: 10.1038/jid.2015.177

Figure 6.

Figure 6

eIF2~P dependent translation repression provides resistance to UVB-induced apoptosis GADD34 overexpressing keratinocytes were treated with 1 μg/mL doxycycline (DOX) for 24 hours to induce GADD34 expression prior to irradiation with 600 J/m2 UVB and characterized by (a) polysome profile analysis, (b) immunoblot analysis, or (c) measurements of caspase-3 specific activity. N-TERTs were pretreated with ISRIB for 1 hour and subsequently irradiated with 600 J/m2 UVB and subjected to (d) polysome profile analysis or (e) caspase-3 specific activity. (f) Keratinocytes expressing GADD34 were treated with vehicle, 24 hours of DOX treatment, 30 minutes 20 μg/mL cycloheximide (CHX) treatment, or a combination of 24 hours DOX followed by 30 minutes CHX. Cells were then irradiated with 0 or 600 J/m2 UVB and assayed for induction of caspase-3 activity. Error bars represent standard deviation (*p<0.01).