. Author manuscript; available in PMC: 2016 Oct 1.

Published in final edited form as: Pharmacol Biochem Behav. 2015 Aug 6;137:53–59. doi: 10.1016/j.pbb.2015.08.003

TABLE 2.

Experimentally determined (ED₅₀mix) and predicted additive (ED₅₀add) values of triazolam + pregnanolone, clonazepam+ganaxolone, triazolam+ganaxolone and clonazepam+pregnanolone mixtures (N=7)

Drug combination	ED₅₀mix(±95% Confidence Interval)	ED₅₀add(±95% Confidence Interval)	Interaction index^{^}
Triazolam/pregnanolone (1:30)	1.718 (1.284–2.152)^*	2.784 (2.066–3.502)	0.63
Triazolam/pregnanolone (1:100)	3.405 (2.118–4.692)^*	5.04 (3.889–6.182)	0.69
Triazolam/pregnanolone (1:300)	5.676 (4.68–6.672)	6.66 (5.257–8.063)	0.87
Clonazepam/ganaxolone (1:10)	1.765 (1.276–2.253)	1.691 (1.212–2.17)	1.07
Clonazepam/ganaxolone (1:30)	5.102 (4.253–5.95)^*	3.141 (2.495–3.787)	1.65
Clonazepam/ganaxolone (1:100)	4.629 (3.407–5.85)	4.725 (4.081–5.369)	0.99
Triazolam/ganaxolone (1:10)	1.981 (1.686–2.275)^*	1.403 (1.159–1.647)	1.47
Triazolam/ganaxolone (1:30)	2.062 (1.738–2.387)^*	2.785 (2.371–3.198)	0.75
Triazolam/ganaxolone (1:100)	4.718 (4.126–5.311)	4.475 (3.94–5.010)	1.06
Clonazepam/pregnanolone (1:10)	1.685 (1.304–2.065)	1.519 (1.131–1.907)	1.3
Clonazepam/pregnanolone (1:30)	1.924 (1.862–1.986)^*	3.125 (2.358–3.892)	0.67
Clonazepam/pregnanolone (1:100)	3.747 (3.034–4.460)	5.307 (4.090–6.524)	0.77

experimentally determined value significantly different than the predictive additive value (p <0.05).

^{^}

interaction index (i.e. ED₅₀mix/ED₅₀add).