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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Invest Dermatol. 2015 May 27;135(10):2475–2483. doi: 10.1038/jid.2015.198

Figure 1. NRAS mutant cell lines express high levels of Plk1 mRNA.

Figure 1

(a) Relative expression of Plk1 in 23 human melanoma cell lines. NRAS mutant melanoma cell lines show higher Plk1 expression compared to non-NRAS and, in particular, to BRAF(V600) mutant cell lines. (b) Plk1 expression in stably transduced primary human melanocytes (PHM) expressing the indicated NRAS mutations or an empty vector control (E). NRAS(Q61) mutant cells show a significant increase of Plk1 expression on mRNA and protein level compared to empty vector controls, non-oncogenic NRAS(N24) mutants and NRAS(G12) mutant cells. Bars represent the mean relative expression of Plk1. β-actin served as an endogenous control. (N=3; * indicates p<0.05; mean±SEM)