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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Bioorg Chem. 2015 Jul 13;62:64–73. doi: 10.1016/j.bioorg.2015.07.003

Figure 3. Kinetic measurements for ghrelin substrates.

Figure 3

Each assay mixture was incubated at 30°C for 1 min in the presence of 50 μM palmitoyl-CoA with 25 μg microsome protein. Solid lines are best-fit to the Michaelis-Menten equation, and Km values are shown in Table 1. (A) Ghrelin27 with 1 μM octanoyl-CoA. (B) Octanoyl-CoA with 10 μM Ghrelin27. (C) Octanoyl-CoA with 80 μM Ghrelin10. (D) Ghrelin10 with 1 μM octanoyl-CoA. (E) Ghrelin10-Tat with 1 μM octanoyl-CoA. (F) Each reaction contained 50 μM of the indicated substrate; Ghr10, Ghrelin10.