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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Invest Dermatol. 2015 Apr 30;135(10):2492–2501. doi: 10.1038/jid.2015.166

Figure 4. PTK6 is activated in UVB damaged skin and UVB-induced skin tumors.

Figure 4

A: PTK6 activation in different cellular locations following long-term UVB treatment. PTK6 PY342 localizes to the cytoplasm in neoplastic cells infiltrating the dermis (left panel). Active PTK6 PY342 is associated with the plasma membrane in the epidermal cell layers (center panels). Membrane and nuclear localization of PTK6 PY342 can be detected in different regions of the same section in endpoint UVB-treated hyperplastic skin showing epidermal necrosis and dermal inflammation (right panel). Size bar = 100 μm. B: Differential localization of active PTK6 in epidermal cells and neoplastic cells in the dermis of spindle cell squamous cell tumors. Tumors that formed in wild type mice were incubated with antibodies against total PTK6 and active PTK6 PY342. In actinic keratosis and anaplastic spindle cell tumors, total PTK6 is expressed throughout the adjacent or overlying epidermis, and PTK6 PY342 is present at the plasma membrane. Both total PTK6 and PTK6 PY342 are found in the cytoplasm of keratin-expressing cells of epithelial origin in the dermis. Keratin 14 expression was detected in suprabasal layers of the hyperplastic skin. Infiltrative neoplastic cells expressed either keratin 14 or keratin 10 identifying them as epithelial in origin. Size bar = 20 μm. C: BrdU incorporation does not correlate with activation of PTK6. BrdU incorporation (red) and membrane associated PTK6 PY342 (FITC, green) does not colocalize in hyperplastic skin or spindle cell squamous cell tumors. Size bar = 20 μm.