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. 2015 Jun 29;185(7):783–796. doi: 10.1007/s00360-015-0917-5

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 8 — Developmental changes in the response of extraembryonic arteries (allantoic and yolk sac) from 15- and 19-day chicken embryos to contractile (a) and relaxant (b) agents. Relaxation to acetylcholine was studied in vessels pre-contracted with 5-HT (1 µM), whereas relaxation to sodium nitroprusside (SNP), isoproterenol and forskolin was studied in vessels pre-contracted with KCI (62.5 mM). Each bar represents the mean + SE of 4–10 embryos. *, **, ***p < 0.05, 0.01, 0.001 for difference (yolk sac vs. allantoic at the same age) in observed or apparent E _max. ^### p < 0.001 for difference (15 vs. 19-days in the same vessel) in apparent E _max