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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Free Radic Biol Med. 2015 May 29;86:191–199. doi: 10.1016/j.freeradbiomed.2015.05.029

Figure 1. Thiobarbituric acid reactive substances (TBARS) in forebrain mitochondria following exposure to carbon monoxide (CO) with or without isoflurane.

Figure 1

Levels of TBARS were determined with a colorimetric assay in mitochondria isolated immediately following exposure. Values from the six experimental cohorts are expressed as means plus standard deviation. (A) TBARS following 1-hour exposure. N = 6 animals per cohort. (B) TBARS following 20 minute exposure. N = 3 animals per cohort. (C) TBARS following 40 minute exposure. N = 3 animals per cohort. (D) TBARS over time. Statistical significance was determined at each time point with two-way ANOVA using post hoc Tukey’s test. One-way ANOVA was utilized to assess significance within each experimental cohort over time. *P < 0.05 vs. CO (or air) matched exposure without isoflurane at same time point. †P < 0.001 vs. air-exposed control at same time point. ‡P < 0.005 vs. air-exposed control at same time point. @P < 0.005 vs. CO matched exposure without isoflurane at same time point. #P < 0.01 vs. CO matched exposure without isoflurane at same time point. ^P < 0.01 vs. cohort matched exposure at 20 and 40 minutes. &P < 0.05 100 ppm CO at 40 minutes vs. cohort matched exposure at 20 minutes.