| 1 cycle: | 1 min | 95°C | (initial denaturation) |
| 25 cycles: | 30 sec | 95°C | (denaturation) |
| 30 sec | 58°C | (annealing) | |
| 2 min | 72°C | (extension) | |
| 1 cycle: | 2 min | 72°C | (final extension) |
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