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. 2015 Jul 24;7:plv086. doi: 10.1093/aobpla/plv086

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Published by Oxford University Press on behalf of the Annals of Botany Company.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Histochemical detection of primary metabolites and carbohydrate-related enzyme activity in the galls of Nothotrioza cattleiani. (A) Reaction of Fehling's reagent to reducing sugars in the chlorophyllous parenchyma (chp), with increased accumulation near the vascular bundles (vb) and tissues near the nymphal chamber (nc). (B) Reaction of Lugol's reagent to starch (arrowheads) in outer cortical cells. (C) Reaction of Coomassie blue to proteins (arrowheads) in a vascular bundle (vb) and perivascular parenchyma. (D) Reaction of Sudan red B to lipids in the oil glands (og) and cuticle (cu) of the outer epidermis. (E and F) Enzyme activity of glucose-6-phosphatase and invertases, respectively, near vascular bundles (vb) and perivascular parenchyma (arrowheads), surrounding the nymphal chamber (nc). Bars: (A) 200 µm; (B) 10 µm; (C) 30 µm; (D–F) 60 µm.