Table S1.
Bacterial strains used in this study
| Strain | Genotype* | Source† |
| BL21 (λDE3) | ompT rB−mB− (PlacUV5::T7gene1) | Novagen |
| BW25113 | lacIq rrnB3 ∆lacZ4787 ∆(araBAD)567 ∆(rhaBAD)568 hsdR514 | (37) |
| BW27783 | BW25113 ∆(araFGH) Φ(∆araEp PCP8−araE) | (38) |
| MG1655 | rph1 ilvG rfb-50 | Laboratory collection |
| MR801‡ | MG1655 mepS-Flag-Kan | MG1655X P1(DY378-mepS-Flag-Kan) |
| MR802 | MR801 (KanS) | MR801/pCP20 |
| MR803 | MR802 ΔnlpI::Kan | MR802 X P1 (ΔnlpI::Kan) |
| MR804 | MR802 Δprc::Kan | MR802 X P1(Δprc::Kan) |
| MR805 | MR802 ΔnlpI::frt | MR803/pCP20 |
| MR806 | MR805Δprc::Kan | MR805 X P1(Δprc::Kan) |
| MR807 | MG1655 ΔphoA::frt ΔmepS::frt | MG1655 ΔphoA ΔmepS::Kan/pCP20 |
| MR808 | MR807 ΔnlpI::Kan | MR807 X P1 (ΔnlpI::Kan) |
| MR809 | MR807 Δprc::Kan | MR807 X P1(Δprc::Kan) |
| MR810 | MG1655 ΔmepS::frt | MG1655 ΔmepS::Kan/pCP20 |
| MR811 | MG1655 ΔnlpI::frt | MG1655 ΔnlpI::Kan/pCP20 |
| MR812 | MG1655 Δprc::frt | MG1655 ΔnlpI::Kan/pCP20 |
| MR813 | MR810 ΔnlpI::frt | MR810 ΔnlpI::Kan/pCP20 |
| MR814 | MR810 Δprc::Kan | MR810 X P1(Δprc::Kan) |
| MR815 | MR811 Δprc::Kan | MR811 X P1(Δprc::Kan) |
| MR816 | BW27783 ΔmepM::frt | BW27783 ΔmepM::Kan/pCP20 |
| MR818‡ | MR810 prc-HA-Cam | MR810 X P1(prc-HA-Cam) |
| MR819‡ | MR810 prcS452A-HA-Cam | MR810 X P1 (prcS452A-HA-Cam) |
| MR820‡ | MR805 prc-HA-Cam | MR805 X P1(prc-HA-Cam) |
| MR821‡ | MR813 prc-HA-Cam | MR813 X P1(prc-HA-Cam) |
| MR822‡ | MR811 prc-HA-Cam | MR811 X P1(prc-HA-Cam) |
*
The deletion alleles mepS (spr), mepM (yebA), and nlpI, phoA were sourced from the Keio collection (37). Deletion mutations were tested for authenticity by linkage, PCR, sequence analysis, and phenotype, if known, and introduced into required strain backgrounds by phage P1 transduction (39).
†
Marker-less (KanS) strains were made by flipping out the Kan cassette using pCP20 that encodes Flp recombinase (40).
‡
The mepS-Flag-Kan, prc-HA-Cam, and prcS452A-HA-Cam constructs are described above in Strain Constructions.