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BNZ132-1-40 inhibits, in a dose-dependent manner, IL-2- and IL-15-mediated proliferation of CTLL-2. (A) Proliferation of CTLL-2 in the presence of IL-2 (10 IU/mL) and various concentrations of BNZ132-1-40. (B) Proliferation of CTLL-2 in the presence of IL-15 (5 ng/mL) and various concentrations of BNZ132-1-40. Proliferation of CTLL-2 was characterized at 24 h of culture with a metabolic assay using WST-1 reagent (Clontech) and quantified by an ELISA reader as the absorbance at 450 nm.
