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The SUMOylated form of the constitutively Pfr phyBTyr276His mutant accumulates to equally high levels in darkness and light. Transgenic phyB-9 seedlings expressing phyB-GFP (WT), phyBLys996Arg-YFP (Lys996Arg), or phyBTyr276His–YFP (Tyr276His) were grown in darkness for 4 d (D) and transferred to WL (50 µmol m−2·s−1) for 24 h. Sample preparation and detection of SUMOylation was performed as described in Fig. 1.
