Fig. S4.
R-induced translocation, accumulation, and complex formation of phyB-YFP and phyBLys996Arg-YFP in the nucleus are identical. (A) Four-day-old seedlings expressing the LIP1:PHYB-YFP (upper row) or the LIP1:PHYBLys996Arg-YFP (lower row) transgene were grown in D and subsequently were irradiated by 1 µmol m−2·s−1 RL for 2 min, 2 h, 6 h, or 24 h. Images using confocal laser scanning microscope were taken on the hypocotyl cells located at the upper part of the hypocotyls. White bars represent 25 µm. (B) Nuclear fluorescence of transgenic seedlings expressing LIP1:PHYB-YFP (white bar) or the LIP1:PHYBLys996Arg-YFP (gray bar) was calculated from confocal laser scanning microscopy images taken of 4-d-old etiolated seedlings treated or not treated with 1 µmol m−2·s−1 RL for 6 h. The ratio of mean gray values derived from nonirradiated/irradiated seedlings from three independent experiments are plotted; error bars indicate the SE.