Fig. S8.

OTS1 binds to phyB in planta. The Agrobacterium cultures containing the combination of constructs (phyB-GFP/HA-OTS1 or GFP/HA-OTS1) were infiltrated into N. benthamiana leaves. Three days after the Agrobacterium infiltration, the expression of the fusion proteins was confirmed by immunoblotting with anti-HA and anti-GFP antibodies (input panels). Coimmunoprecipitation assays performed with anti-GFP antibodies showed that HA-OTS1 immunoprecipitated with phyB-GFP but not with GFP alone, indicating that OTS1 interacts with phyB.