Fig. 2.

In vivo effect of VIS410 and oseltamivir in a BALB/c mouse model of H3N2. Animals were infected intranasally with a lethal dose of mouse-adapted influenza A/Victoria/3/75 virus (2 LD₅₀). Mice were weighed daily to day 15, or until death, and then once again on day 19. (A) Five groups of 10 mice each were administered VIS410 at 2.5 (blue diamonds), 5 (red diamonds), 10 (orange triangles), or 20 (green squares) mg/kg i.p. 48 h after virus exposure; 15 mice received placebo (black circle). Mice were euthanized on the day that 30% weight loss was observed. Statistical inferences were made by two-way ANOVA, followed by pairwise comparisons to compare each treatment group with the placebo group, considering the survival of both (Tukey’s multiple comparisons tests). VIS410 had a significant effect on survival (P < 0.001). (B) The combination of VIS410 at 5 mg/kg and oseltamivir at 10 mg/kg daily (green squares) had a greater effect on preventing weight loss than did 5 mg/kg VIS410 (red diamonds) or oseltamivir at 10 mg/kg daily (brown triangles) alone (*P < 0.001). The placebo is also shown (black circle).