Fig. 4.
Loss of miR-196 function alters global Hox signatures. (A) Extensive Hox gene dysregulation is identified following loss of miR-196. Quantitative expression analysis of all 39 Hox genes in cells isolated from E9.5 mutant embryos; genotype comparisons are color coded. Hox genes with one or more predicted miR-196 target binding sites are indicated in red. Filled circles at the tips of fold changes represent a statistically significant change at q < 0.05. (B and C) WISH analysis of miR-196a2GFP/GFP;miR-196b−/− E9.5 embryos relative to WT identifies a caudal expansion of Hoxb8 (B; n = 3/3) and Hoxc8 (C; n = 3/3). The PSM is indicated with a red line/arrowhead and neural tube with a white arrowhead.