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Generation of miR-196a1GFP and miR-196a2GFP knock-in mouse lines. (A and B) Knock-in targeting strategy for miR-196a1GFP (A) and miR-196a2GFP (B). (C and D) Correct targeting was confirmed by Southern blot analysis of the miR-196a1 (C) and miR-196a2 (D) loci. The Southern blot strategy for each knock-in allele is indicated (A and B), with the position of each Southern probe indicated with a blue box.
