Table 1.
Primers employed in this study.
| Genes | Primers (5′-3′) | Annealing temperature (°C) | PCR products (bp) |
|---|---|---|---|
| embC | F: GATACCCGCTACAGCAGCA | 63 | 334 |
| R: GGTCGTAGTACCAGCCGAAA | |||
|
| |||
| embA1 | F: GCCGGCTATGTAGCCAACTA | 63 | 338 |
| R: GACCGTTCCACCAACACC | |||
|
| |||
| embA2 | F: GCGCGCTGGACATCTCGAT | 68 | 704 |
| R: CGCCTCCGTCGTGCCGAAATA | |||
|
| |||
| embB1 | F: CCGACCACGCTGAAACTGC | 63 | 364 |
| R: GTAATACCAGCCGAAGGGATCCT | |||
|
| |||
| embB2 | F: GACGGCTACATCCTGGGCATG | 68 | 525 |
| R: TGCCGACCAGGCGATGACG | |||
|
| |||
| embB3 | F: CGTCATCGCCTGGTCGGCAC | 64 | 812 |
| R: ACATGGTGCCGAAGATGACGC | |||
|
| |||
| embR | F: CGCTGATCTGGAACGTGAAT | 65 | 1137 |
| R: GTAGCGCGACAGTGGAGAAG | |||
|
| |||
| ubiA | F: TGACTCAACCTCCGGCAAACC | 63 | 850 |
| R: GCGCCAGCAGCTGCAATACCC | |||