Table 2.
Characteristics of the mutants in embB, embA, embC, embR, and ubiA within the EMB-resistant isolates.
| Types | Locus, nucleotide change, and amino acid change | Number (n = 109) | High resistance (n = 37) |
Resistant spectrum (mean ± SD = 3.49 ± 1.59) |
MDR/XDR | ||||
|---|---|---|---|---|---|---|---|---|---|
| embB | embA | embC | embR | ubiA | |||||
| 1 | WT | WT | WT | WT | WT | 14 | 0 | 1.8 | 6 none, 8 MDR |
| 2 | M306V (ATG-GTG) | WT | WT | WT | WT | 22 | 5 | 1.5 | 9 none, 13 MDR |
| 3 | M306L (ATG-CTG) | WT | WT | WT | WT | 8 | 3 | 1.9 | 2 none, 6 MDR |
| 4 | M306L (ATG-TTG) | WT | WT | WT | WT | 2 | 1 | 2.2 | 1 none, 1 MDR |
| 5 | M306I (ATG-ATA) | WT | WT | WT | WT | 8 | 2 | 3.8 | 3 none 4 MDR, 1 XDR |
| 6 | M306I (ATG-ATT) | WT | WT | WT | WT | 1 | 0 | 4 | 1 MDR |
| 7 | M306I (ATG-ATT) | WT | WT | WT | E149D (GAA-GAC) | 1 | 1 | 8 | 1 XDR |
| 8 | M306I (ATG-ATA) G406S (GGC-AGC) |
WT | WT | WT | WT | 1 | 1 | 5 | MDR |
| 9 | M306I (ATG-ATA) G406D (GGC-GAC) |
WT | WT | WT | WT | 1 | 1 | 4 | MDR |
| 10 | M306I (ATG-ATA) G406D (GGC-GAC) |
WT | WT | 462 (c-del) | V49L (GTC-CTC) P254P (CCG-CCT) |
1 | 1 | 4 | MDR |
| 11 | M306I (ATG-ATC) R354S (AGA-AGC) |
V122G (GTG-GGG) V125G (GTG-GGG) |
WT | WT | WT | 1 | 1 | 4 | MDR |
| 12 | M306V (ATG-GTG) | V343L (GTG-TTG) | WT | WT | WT | 1 | 0 | 6 | MDR |
| 13 | M306V (ATG-GTG) | WT | E305D (GAG-GAC) | WT | WT | 1 | 1 | 7 | XDR |
| 14 | M306V (ATG-GTG) | WT | WT | G84G (GGT-GGG) S104N (AGC-AAC) |
A38T (GCC-ACC) | 1 | 1 | 5 | MDR |
| 15 | M306V (ATG-GTG) | WT | WT | WT | L198L (CTG-CTC) | 1 | 1 | 8 | XDR |
| 16 | M306V (ATG-GTG) | WT | WT | WT | I206I (ATC-ATT) | 1 | 0 | 3 | None |
| 17 | M306V (ATG-GTG) | L105V (CTG-GTG) | WT | WT | WT | 1 | 0 | 7 | XDR |
| 18 | M306V (ATG-GTG) | R380P (CGT-CCT) | WT | WT | WT | 1 | 0 | 3 | MDR |
| 19 | M306V (ATG-GTG) D328H (GAT-CAT) |
WT | WT | WT | WT | 1 | 0 | 3 | MDR |
| 20 | M306V (ATG-GTG) D328G (GAT-GGT) |
WT | WT | WT | WT | 1 | 1 | 6 | MDR |
| 21 | M306V (ATG-GTG) G406P (GGC-CCG) A439A (GCA-GCG) |
WT | WT | WT | E149D (GAA-GAC) | 1 | 1 | 7 | XDR |
| 22 | M306V (ATG-GTG) G406P (GGC-CCG) A439A (GCA-GCG) |
WT | WT | WT | I206I (ATC-ATT) | 1 | 1 | 6 | MDR |
| 23 | M306V (ATG-GTG) G406A (GGC-GCC) |
WT | WT | WT | WT | 1 | 1 | 3 | MDR |
| 24 | M306V (ATG-GTG) Q497R (CAG-CGG) |
WT | E305E (GAG-GAA) | WT | WT | 1 | 1 | 4 | MDR |
| 25 | M306V (ATG-GTG) D531D (GAC-GAT) |
WT | WT | WT | WT | 4 | 1 | 5.5 | 2 none, 2 MDR |
| 26 | D328H (GAT-CAT) | WT | WT | WT | WT | 1 | 0 | 4 | MDR |
| 27 | R354S (AGA-AGC) | WT | WT | WT | WT | 3 | 0 | 2 | 1 none, 2 MDR |
| 28 | R354S (AGA-AGC) D531D (GAC-GAT) |
WT | WT | WT | WT | 1 | 0 | 3 | None |
| 29 | G406P (GGC-CCG) | WT | WT | WT | WT | 1 | 1 | 7 | XDR |
| 30 | G406P (GGC-CCG) A439A (GCA-GCG) |
WT | WT | WT | WT | 2 | 0 | 5.5 | MDR |
| 31 | G406D (GGC-GAC) | WT | WT | WT | WT | 4 | 2 | 5 | 3 MDR, 1 XDR |
| 32 | G406A (GGC-GCC) | WT | WT | WT | WT | 6 | 2 | 5.5 | 5 MDR, 1 XDR |
| 33 | G406A (GGC-GCC) | WT | E305E (GAG-GAA) | WT | WT | 1 | 0 | 5 | MDR |
| 34 | G406C (GGC-TGC) | WT | WT | WT | WT | 1 | 1 | 4 | MDR |
| 35 | A439A (GCA-GCG) | WT | WT | WT | WT | 2 | 0 | 3.5 | 2 none |
| 36 | Q497R (CAG-CGG) | WT | WT | WT | WT | 5 | 3 | 5.4 | 2 none, 2 MDR, 1 XDR |
| 37 | Q497K (CAG-AAG) | WT | WT | WT | WT | 1 | 1 | 6 | MDR |
| 38 | Q497R (CAG-CGG) T496N (ACC-AAC) |
WT | WT | WT | I179T (ATC-ACC) | 1 | 1 | 7 | XDR |
| 39 | Q497R (CAG-CGG) | WT | WT | P49A (CCC-GCC) | WT | 1 | 1 | 6 | None |
| 40 | WT | WT | WT | P49A (CCC-GCC) P243S (CCC-TCC) |
WT | 1 | 0 | 4 | MDR |
| 41 | WT | WT | WT | WT | S244T (AGC-ACC) | 1 | 0 | 5 | MDR |
WT: wild type; MDR: multidrug resistance; XDR: extensively drug resistance.