Author response image 1.
lnvestigating whether deleting R2 or B alters colP of þcat with Axin in SW480 cells. (A) Expression of Flag-Axin with indicated GFP-tagged APC2 constructs followed by lP of Flag-Axin and examination of the level of ßcat that colPs. Representative immunoblot of 3 independent experiments. (B) Quantification of 3 independent co-lPs. þcat levels were normalized to Flag-Axin. Levels of colPed ßcat were higher in 2 of 3 experiments with APC26R2 and in all three experiments with APC26B.