Table 1.
Quantitation of relative expression levels of transfected versus endogenous APC and Axin
| Summary Axin overexpression | |||
|---|---|---|---|
| Experiment | I | II | III |
| Ratio GFP-FlyAxin to GFP-hAxin1 | 1.04 | 0.11 | 0.48 |
| Ratio GFP-hAxin1 to endo hAxin1 | 33.87 | 431.67 | 62.82 |
| Transfection efficiency | 30% | 42% | 36% |
| Fold overexpression level of GFP-FlyAxin to endo hAxin1 | 117.41 | 113.06 | 83.76 |
| Summary APC2 overexpresssion | |||
|---|---|---|---|
| Experiment | I | II | III |
| Ratio Flag-FlyAPC2 to Flag-hAPC1-1338 | 17.31 | 138.45 | 28.02 |
| Ratio Flag-hAPC1-1338 to endo hAPC1-1338 | 25.17 | 5.86 | 14.69 |
| Transfection efficiency | 30% | 33% | 25% |
| Fold overexpression level of Flag-FlyAPC2 to endo hAPC1-1338 | 1452.30 | 2458.54 | 1646.46 |