Figure 2.

MPIO-αVCAM-1 enhanced MRI allows semi-quantitative measurement of endothelial activation in the kidneys with a temporal resolution of 24 hours. (A) Experimental design. (B) Representative high-resolution T2*-weighted images without (top) and with magnification (bottom) from mice treated with either saline or different amounts of LPS (from 0.1 to 0.8 mg/kg). (C) Corresponding quantification (n=3/group). *p<0.05 vs control/saline. (D) Experimental design. (E) Representative consecutive slices (from a single mouse) from high-resolution T2*-weighted images before and at different time after MPIO-aVCAM-1 administration in LPS mice (1 mg/kg). This allows to appreciate the kinetic of MPIO-αVCAM-1 clearance from the inflamed kidneys 48 hours after LPS administration. Signal voids decreased gradually with time after MPIOs-αVCAM-1 injection. (F) Corresponding quantification (n=3/group).