Figure 6. Immunodetection of osteogenic differentiation markers: effect of ODM treatment and PEMF exposure for BM-hMSCs grown up to 27 days.
Localization of specific osteogenic markers, alkaline phosphatase, collagen type I, decorin and osteopontin, performed on the same samples as in Fig. 5E. Staining confirmed that the osteogenic phenotype is triggered by ODM treatment (columns 2 and 4) and is maintained by PEMF exposure (column 4) or even slightly incremented (see osteopontin).