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. 2015 Sep 14;5:13987. doi: 10.1038/srep13987

Figure 1. Magnetic 3D bioprinting.

Figure 1

(a) Cells grown to 70-80% confluence in 2D were incubated overnight with magnetic nanoparticles (NanoShuttle, NS). After resuspending and levitating the cells for a few hours, the cells and ECM were distributed evenly into the wells of a 96-well plate. The cells were then printed for 15 min by putting the plate atop a 96-well magnetic drive. After printing, the magnet was removed and the spheroid contracts. (b) Spheroid contraction over 6 h as cells rearrange and compact. (nuclei = blue). (c) The mobile device-based imaging system, with the 96-well plate full of spheroids placed above the mobile device, which was set to image the whole plate at programmed intervals as short as 1 s. Scale bar = 250 μm.