Figure 5.

Structure and Expression of the Bige1 Gene.

(A) bige1 mutations including four deletion alleles and two Mu insertion alleles are shown in the context of Bige1 locus structure. Black and gray boxes represent protein coding exons and untranslated regions, respectively.

(B) RT-qPCR analysis of Bige1 expression in various tissues of maize. Seven-day-old seedlings were dissected into indicated tissues including three regions of the primary root: lateral root (LR) zone, elongation zone, and root tip. Values were normalized to 18S rRNA, and expression in the 1st leaf of the seedlings was set to 1. The values are means of technical duplicate assays.

(C) Unrooted tree of the subfamily of BIGE1-related MATE transporters from maize, rice, and Arabidopsis constructed using MEGA6 (Tamura et al., 2013) with a bootstrap value of 1000. The N-terminal variable regions of MATE proteins were removed for the phylogenic analysis (Supplemental Data Set 1). Characterized proteins including maize BIGE1 (GRMZM2G148937) and Arabidopsis ADP1 (AT4G29140) and ZRZ/BCP1 (At1G58340) are highlighted in bold.